mmps 1 timp 1  (Boster Bio)

Journal: Current Issues in Molecular Biology

Article Title: Silencing LY6K Suppresses CD44 + EpCAM + HCT116 Human Colon Cancer Stem Cells Growth: Insights from In Vitro and In Vivo Evidence

doi: 10.3390/cimb46120840

Figure Lengend Snippet: Knockdown of LY6K inhibits migration and invasion of CCSCs in vitro. Optical micrographs and the number of migrated ( A ) and invaded ( B ) CCSCs following siLY6K or siNC transfection for 48 h according to Transwell migration and invasion assay. n = 3. ( C ) Western blot analyses showing the protein levels of MMP-2, MMP-9, and TIMP-1 in CCSCs after treatment with siLY6K or siNC for 48 h. Values are the mean ± SE (n = 3). ** p < 0.01, *** p < 0.001, or **** p < 0.0001 indicates significant differences from the Mock and siNC group as assessed by one-way ANOVA with Tukey–Kramer multiple comparisons tests. LY6K, lymphocyte antigen 6, locus K; CCSC, colon cancer stem cells; MMP, matrix metalloproteinase; TIMP-1, tissue inhibitor of MMP-1; n.s., not significant.

Article Snippet: The membranes were incubated overnight at 4 °C with primary antibodies [LY6K (1:750, #PIPA572689, Invitrogen), MMP-2 (3:1000, #BM0569, Boster, Pleasanton, CA, USA), MMP-9 (1:300, #BM4089, Boster), tissue inhibitor of MMPs-1 (TIMP-1) (3:1000, #BM4980, Boster), cyclin-dependent kinase (CDK) 4 (3:1000, #BM4672, Boster), cyclin D1 (3:1000, #bs-0623R, Bioss), CDK2 (3:1000, #bs-0757R, Bioss), cyclin E1 (3:1000, #bs-0573R, Bioss), caspase-3 and cleaved caspase-3 (1:1000, #14220, CST), β-actin (1:1500, #AF0003, Beyotime), and β-tubulin (1:1000, #AC008, Beyotime)], followed by incubation with secondary antibodies [horseradish peroxidase-conjugated goat anti-rabbit (1:2000, #A0208, Beyotime) and anti-mouse (1:2000, #A0216, Beyotime)] for 1 h at room temperature.

Techniques: Knockdown, Migration, In Vitro, Transfection, Invasion Assay, Western Blot

Journal: iScience

Article Title: Human monocyte-derived macrophages shift subcellular metalloprotease activity depending on their activation state

doi: 10.1016/j.isci.2024.111171

Figure Lengend Snippet:

Article Snippet: Human MMP-9/TIMP-1 DuoSet ELISA , R&D Systems , Cat # DY1449.

Techniques: Plasmid Preparation, Recombinant, Knock-Out, Blocking Assay, Staining, Antibody Labeling, Reverse Transcription, Lysis, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Fractionation, Cell Culture, Software, Flow Cytometry, Fluorescence, Spectrophotometry

Journal: Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale

Article Title: Proteolytic system parameters in the brain of rats with hyperhomocysteinemia

doi: 10.4081/jbr.2024.12232

Figure Lengend Snippet: Figure 2. Levels of matrix metalloproteinases and tissue inhibitor of metalloproteinases in the brain of rats with hyperhomocysteinemia. Values are expressed as mean ± SEM (n=10); *p<0.05 compared to the control group of corresponding age; #p<0.05 compared to the group “Young_Control”.

Article Snippet: The following reagents were used in our research: DL-HTL hydrochloride (Acros Organics, Italy); casein, trichloroacetic acid (TCA), ethylenediaminetetraacetic acid (EDTA), phenylmethylsulfonyl fluoride (PMSF), hydrogen peroxide and o-phenylenediamine (Sigma-Aldrich, St. Louis, MO, USA); anti-rat monoclonal antibodies to cytokines, tissue inhibitor of metalloproteinases-1 (TIMP-1) and MMPs (Santa Cruz Biotechnology, Inc., Dallas, Texas, USA); horseradish peroxidase-conjugated secondary antibodies (SigmaAldrich, St. Louis, MO, USA).

Techniques: Control